Samurai X: Trust and Betrayal OVA

(click on thumbnails to get bigger capture)
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Takasugi Shinsaku with Shamisen discussing ...with Katsura Kogoro the state of affairs, which included the taking on of a young assassin for his purposes.
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Takasugi Shinsaku extracts a promise from Katsura Kogoro (later Kido Takayoshi) to never again lift his sword, and to remain pure. Katsura agrees.
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On one of his many journeys between his home province and Kyoto where he serves. Ikumatsu (later Kido Matsuko), the geisha of Gion, who saved his life when he had to flee Kyoto after the unsuccessful attempt at Hamahuri Gate.
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  Takasugi thinking...
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Actually, we get hints at his illness, and what is to come. Takasugi in prison.